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Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

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Related Experiment Video

Updated: Jun 21, 2026

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

Published on: May 25, 2015

ABO genotyping by single strand conformation polymorphism--using CE.

James Chun-I Lee¹, Hsing-Mei Hsieh, Hsiao-Feng Teng

¹Department of Forensic Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.

Electrophoresis

|July 30, 2009

Summary

This summary is machine-generated.

This study introduces a novel ABO genotyping method using PCR-SSCP and CE, enabling rapid and accurate ABO blood group determination from degraded samples. This technique is valuable for clinical transfusion and forensic applications.

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An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations

Published on: November 3, 2010

Area of Science:

Molecular Biology
Genetics
Forensic Science

Background:

Accurate ABO blood group genotyping is crucial for transfusion medicine and forensics.
Existing methods may struggle with degraded or trace DNA samples.

Purpose of the Study:

To develop and validate a novel, rapid ABO genotyping method.
To enable accurate ABO blood group determination from challenging samples.

Main Methods:

Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) to generate amplicons.
Capillary Electrophoresis (CE) for amplicon separation and analysis.
Utilized four amplicons targeting exons 6 and 7 of the ABO gene.

Main Results:

Successfully identified ABO alleles including A(1), A(1v), B, O(1), O(1v), and O(2).
Demonstrated high precision with low standard deviation (≤0.37) and minimal mobility variation (0.05–0.39).
The method effectively distinguishes alleles with 1-4 SNPs due to wide mobility differences (1.88–12.01).

Conclusions:

The developed PCR-SSCP and CE method provides a robust and efficient approach for ABO genotyping.
Its ability to analyze small amplicons (up to 160 bp) makes it ideal for degraded DNA.
The method holds significant potential for clinical transfusion and forensic investigations.