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Related Experiment Video

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Assessment of Vascular Tone Responsiveness using Isolated Mesenteric Arteries with a Focus on Modulation by Perivascular Adipose Tissues
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NTPDase1 (CD39) controls nucleotide-dependent vasoconstriction in mouse.

Gilles Kauffenstein1, Annick Drouin, Nathalie Thorin-Trescases

  • 1Centre de Recherche en Rhumatologie et Immunologie, Université Laval, Québec, QC, Canada G1V 4G2.

Cardiovascular Research
|July 31, 2009
PubMed
Summary
This summary is machine-generated.

The ectonucleotidase NTPDase1 is crucial for regulating nucleotide levels in blood vessels. Mice lacking NTPDase1 show enhanced vasoconstriction, highlighting its role in vascular tone control.

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Area of Science:

  • Vascular Biology
  • Biochemistry
  • Pharmacology

Background:

  • Extracellular nucleotides act as vasoactive molecules.
  • Ectonucleotidases regulate extracellular nucleotide concentrations.
  • NTPDase1 is a key ectonucleotidase in blood vessels.

Purpose of the Study:

  • To investigate the role of NTPDase1 in nucleotide-mediated vasoconstriction.
  • To utilize Entpd1(-/-) mice to study NTPDase1 function in vivo and in vitro.
  • To elucidate the mechanisms underlying nucleotide-induced vascular responses.

Main Methods:

  • Immunofluorescence and enzyme histochemistry to assess NTPDase expression and activity.
  • Vascular reactivity studies in isolated arteries and in vivo blood pressure measurements.
  • RT-PCR to determine nucleotide receptor expression in vascular smooth muscle cells (VSMCs).

Main Results:

  • Entpd1(-/-) mice exhibited significantly reduced nucleotidase activity in blood vessels and VSMCs.
  • UDP and UTP caused potent, long-lasting vasoconstriction in aortic rings from Entpd1(-/-) mice via P2Y(6) receptor activation.
  • UDP infusion potentiated blood pressure increases in Entpd1(-/-) mice, and enhanced myogenic response was observed in their mesenteric arteries.

Conclusions:

  • NTPDase1 is the primary enzyme regulating nucleotide metabolism on VSMCs.
  • NTPDase1 plays a critical role in the local control of vascular tone by extracellular nucleotides.
  • Targeting NTPDase1 could offer therapeutic strategies for vascular tone modulation.