Quantification of D-Asp and D-Glu in rat brain and human cerebrospinal fluid by microchip electrophoresis
Yong Huang1, Ming Shi, Shulin Zhao
1College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, China.
Abstract:
A microchip electrophoresis (MCE) method with LIF detection was presented for quantification of D-aspartic acid (D-Asp) and D-glutamate (D-Glu) in biological samples. D-Asp and D-Glu were determined after precolumn derivatization with FITC. The chiral separation was performed on a glass/PDMS hybrid microfluidic chip using gamma-CD as chiral selector in the running buffer. High sensitive detection was obtained by the LIF detection. The LODs (S/N = 3) for D-Asp and D-Glu were 6.0x10(-8) and 4.0x10(-8) M, respectively. Using this method, the levels of D-Asp and D-Glu in rat brain and human cerebrospinal fluid (CSF) were determined.
