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Determination of chloramphenicol in urine, feed water, milk and honey samples using molecular imprinted polymer
Martina Rejtharová1, Libor Rejthar
1Institute for State Control of Veterinary Biologicals and Medicaments, CZ 621 00 Brno, Czech Republic. rejtharova@uskvbl.cz
Abstract:
A method for determination of low concentrations of chloramphenicol in urine, feed water, milk and honey was developed. A comparison was carried out between a routinely used analytical method based on solid phase extraction (SPE-C18) for cleaning the extract and the new procedure for the sample preparation using columns based on the molecular imprinted polymers (MIP) principle. The extracts obtained from the MIP clean-up procedure were clean enough for chromatografic analyses. Confirmatory analyses were conducted using GC/MS-NCI after derivatisation (silylation). The described method was fully validated according to CD 2002/657/EC. This method is considerably robust and allows very dirty samples to be processed. The described MIP procedure is very simple and low-time-consuming, and provides high throughput of the samples examined. This could be used for routine screening and confirmatory analyses as well.
