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Updated: Jun 21, 2026
08:32
Conducting Multiple Imaging Modes with One Fluorescence Microscope
Published on: October 28, 2018
Wide-field extended-resolution fluorescence microscopy with standing surface-plasmon-resonance waves
Euiheon Chung1, Yang-Hyo Kim, Wai Teng Tang
1Department of Mechanical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
Optics Letters
|August 4, 2009
Summary
A new microscopy technique called standing-wave surface-plasmon-resonance fluorescence (SW-SPRF) overcomes light diffraction limits. This advanced optical imaging offers nanoscale resolution for sensitive biomolecular detection and other applications.
Area of Science:
- Optics and Photonics
- Nanotechnology
- Biophysics
Background:
- Conventional surface-plasmon-resonance (SPR) imaging resolution is limited by light diffraction.
- Need for advanced imaging techniques with higher resolution in nanoscale research.
Purpose of the Study:
- To develop a wide-field extended-resolution optical imaging technique.
- To overcome the diffraction limit in SPR imaging.
- To enable sensitive biomolecular detection and nanoscale imaging.
Main Methods:
- Development of standing-wave SPR fluorescence (SW-SPRF) microscopy.
- Utilizing evanescent SPR standing waves for imaging.
- Wide-field optical imaging approach.
Main Results:
- Achieved lateral resolution approaching 100 nm.
- Demonstrated significant signal enhancement.
- Showcased background-noise reduction capabilities.
Conclusions:
- SW-SPRF microscopy offers extended resolution beyond conventional SPR.
- The technique provides enhanced sensitivity and reduced noise for imaging.
- SW-SPRF has potential applications in biomolecular detection, nanoscale imaging, and lithography.
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