Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Functional Divergence and Structural Changes of class IV Histone Deacetylases (HDACs) Across the Tree of Life.

Molecular biology and evolution·2026
Same author

Macrophage-Centric Phenotypic Screening Identifies Tetrazolone-Based HDAC6 Inhibitors That Reprogram the Tumor Immune Microenvironment and Improve Immune Checkpoint Blockade.

Journal of medicinal chemistry·2026
Same author

Beyond formic acid: Peptides in carpenter ant venoms aid in disease protection.

Science advances·2026
Same author

Capsaicin-Inspired Hydroxamate Hybrids as Selective HDAC6 Inhibitors with Antiproliferative Activity in Hematological Malignancies.

ACS omega·2026
Same author

Selective targeting of cortactin tandem repeat acetylation by human lysine deacetylases.

The FEBS journal·2026
Same author

Total Synthesis and Structural Revision of Keenamide A.

Journal of natural products·2026
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Jun 21, 2026

Identifying Protein-protein Interaction Sites Using Peptide Arrays
07:44

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

Peptide arrays for enzyme profiling.

Alexandra Thiele1, Johannes Zerweck, Mike Schutkowski

  • 1Max Planck Research Unit for Enzymology of Protein Folding, Halle, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|August 4, 2009
PubMed
Summary
This summary is machine-generated.

Peptide microarrays enable rapid profiling of enzyme substrate specificity for kinases, proteases, and phosphatases. This technology aids in identifying enzyme targets and optimizing assays for biological research.

More Related Videos

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays
08:48

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

Published on: November 29, 2014

Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays
07:42

Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays

Published on: September 19, 2018

Related Experiment Videos

Last Updated: Jun 21, 2026

Identifying Protein-protein Interaction Sites Using Peptide Arrays
07:44

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays
08:48

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

Published on: November 29, 2014

Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays
07:42

Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays

Published on: September 19, 2018

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Enzymology

Background:

  • Enzymes, including kinases, proteases, and phosphatases, are crucial for signal transduction.
  • Characterization of the vast majority of human enzymes remains incomplete.
  • Enzymatic activity patterns can serve as biomarkers for cellular or organismal states.

Purpose of the Study:

  • To provide a comprehensive overview of enzyme profiling using peptide microarrays.
  • To highlight the principles and advantages of peptide microarray technology for enzyme characterization.
  • To discuss the application of this technology in high-throughput screening (HTS) and target identification.

Main Methods:

  • Utilizing peptide microarray-based technologies for high-throughput enzyme substrate specificity profiling.
  • Analyzing patterns of enzymatic activities to fingerprint biological states.
  • Focusing on assay principles for enzyme profiling.

Main Results:

  • Peptide microarrays offer extremely efficient profiling of enzyme substrate specificity.
  • This technology significantly reduces assay set-up time for HTS.
  • It enables the identification of downstream enzyme targets and optimization of enzyme substrates.

Conclusions:

  • Peptide microarrays represent a powerful tool for advancing enzyme characterization and functional studies.
  • The technology facilitates a deeper understanding of enzyme roles in biological pathways.
  • Enzyme profiling via peptide microarrays has broad implications for drug discovery and diagnostics.