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Generation and Culture of Blood Outgrowth Endothelial Cells from Human Peripheral Blood
Published on: December 23, 2015
On the origin of (CD105+) circulating endothelial cells
Michiel H Strijbos1, Cornelis Verhoef, Jan W Gratama
1Erasmus University Medical Center - Daniel den Hoed Cancer, Center Groene Hilledijk 301, 3075 EA Rotterdam, The Netherlands. m.strijbos@erasmusmc.nl
Thrombosis and Haemostasis
|August 5, 2009
Summary
The CellSearch assay effectively detects circulating endothelial cells (CECs) from damaged vasculature, not bone marrow-derived cells. This finding supports the assay's use in non-malignant vascular disorders.
Area of Science:
- Vascular biology
- Cellular immunology
- Biomarker discovery
Background:
- Circulating endothelial cells (CECs) are typically associated with vascular damage.
- The CellSearch assay identifies CECs using CD146, CD105, and CD45 markers.
- Uncertainty exists regarding CEC origin (vascular vs. bone marrow) and CD105 expression in non-malignant conditions.
Purpose of the Study:
- To investigate if the CellSearch assay detects CECs from damaged vasculature or endothelial progenitor cells (EPCs).
- To determine the suitability of the CellSearch assay for non-malignant diseases.
- To assess CD105 expression on CECs from healthy and damaged vasculature.
Main Methods:
- CEC counts were measured in patients undergoing isolated limb perfusion and healthy donors.
- Vascular damage was induced via venesection and cannulation.
- Tumor necrosis factor (TNF) was administered to assess CEC integrity.
Main Results:
- CEC counts significantly increased following venesection and cannulation, indicating vascular origin.
- Non-intact CECs were observed after TNF administration, suggesting apoptosis.
- CD105 expression was confirmed on CECs from damaged normal vasculature.
Conclusions:
- The CellSearch assay detects CECs originating from vascular damage.
- CD105 is expressed on CECs from damaged normal vasculature, validating its use in non-malignant conditions.
- Further research into CECs detected by this assay is warranted for vascular damage assessment.
