Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400 keV in...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A preoperative Artificial Intelligence model to estimate cancer-specific mortality in nonmetastatic kidney cancer patients.

Nature communications·2026
Same author

Mitochondrial calcium uptake drives organelle remodeling to promote inflammasome-dependent cytokine release.

Cell death and differentiation·2026
Same author

Real-world insights into coronary CTA prognostication: value of semiquantitative scores.

La Radiologia medica·2026
Same author

Advancing Italian biophysics: insights from the 27th SIBPA congress.

European biophysics journal : EBJ·2026
Same author

Deruxtecan-based antibody-drug-conjugates induce senescence in HER2-positive breast cancer.

Scientific reports·2026
Same author

Automatic CT-based quantification of myocardial extracellular volume fraction and high ECV burden and their prognostic value for death and heart failure in patients with aortic stenosis: a real-world data analysis.

Journal of cardiovascular computed tomography·2026
Same journal

Seeing, Interpreting, and Trusting Images in the Age of Artificial Intelligence.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
Same journal

P63 and Tight Junctions Targeted for Therapy in Human Salivary Duct Adenocarcinoma Cells.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
Same journal

Human iPSC-Derived Blood Vessel Organoids for Studying Chronic Hypoxia-Induced Microvascular Dysfunction.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
Same journal

From Manual to Macro: A Reproducible Fiji Workflow for Semi-automated Collagen Fibril Diameter Quantification in Transmission Electron Microscopy.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
Same journal

ADAMTS1 Is Required for Ventral Abdominal Wall Closure.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
Same journal

FGF2 Boost for Driving Forebrain Organoid Maturation Under Static Conditions.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
See all related articles

Related Experiment Video

Updated: Jun 21, 2026

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
11:16

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Published on: August 7, 2016

Advanced correlative light/electron microscopy: current methods and new developments using Tokuyasu cryosections.

Katia Cortese1, Alberto Diaspro, Carlo Tacchetti

  • 1Centro di Ricerca MicroSCoBio, Genoa, Italy.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
|August 6, 2009
PubMed
Summary
This summary is machine-generated.

Correlative light and electron microscopy (CLEM) bridges the resolution gap between light and electron microscopy. Advanced CLEM techniques offer high-resolution 3D imaging for cellular structures and protein localization.

More Related Videos

Low-Cost Cryo-Light Microscopy Stage Fabrication for Correlated Light/Electron Microscopy
10:00

Low-Cost Cryo-Light Microscopy Stage Fabrication for Correlated Light/Electron Microscopy

Published on: June 5, 2011

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
13:43

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Published on: June 24, 2013

Related Experiment Videos

Last Updated: Jun 21, 2026

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
11:16

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Published on: August 7, 2016

Low-Cost Cryo-Light Microscopy Stage Fabrication for Correlated Light/Electron Microscopy
10:00

Low-Cost Cryo-Light Microscopy Stage Fabrication for Correlated Light/Electron Microscopy

Published on: June 5, 2011

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
13:43

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Published on: June 24, 2013

Area of Science:

  • Cell Biology
  • Microscopy Techniques
  • Biomolecular Imaging

Background:

  • Light microscopy (LM) excels at live-cell imaging and protein tracking.
  • Electron microscopy (EM) provides superior ultrastructure and protein labeling resolution.
  • A gap exists between LM and EM in resolving cellular details.

Purpose of the Study:

  • To overview major correlative light and electron microscopy (CLEM) methods.
  • To highlight recent advancements in CLEM.
  • To focus on cryosection-based CLEM techniques for biomolecular imaging.

Main Methods:

  • Correlative Light and Electron Microscopy (CLEM)
  • Immuno-electron microscopy (Immuno-EM)
  • Electron Tomography (ET) with rapid freezing
  • Cryosectioning for combined imaging

Main Results:

  • CLEM strategies bridge the resolution gap between LM and EM.
  • Hybrid immuno-EM techniques offer sensitive protein detection and high-resolution localization.
  • 3D analysis is enhanced by ET and rapid freezing in CLEM.

Conclusions:

  • CLEM provides powerful tools for quantitative 3D analysis of cellular ultrastructure and protein localization.
  • Cryosection-based CLEM advances combined biomolecular imaging.
  • Future developments promise further integration and resolution improvements in CLEM.