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Quantifying noise in optical tweezers by allan variance
Fabian Czerwinski1, Andrew C Richardson, Lene B Oddershede
1Niels Bohr Institute, University of Copenhagen Blegdamsvej 17, 2100 København Ø, Denmark. czerwinski@nbi.dk
Optics Express
|August 6, 2009
Summary
Allan variance offers a simple method to quantify noise in optical tweezers, optimizing experiments and improving single-molecule assay analysis. This technique helps determine ideal measurement parameters and assess environmental noise effects.
Area of Science:
- Physics
- Biophysics
- Nanotechnology
Background:
- Optical tweezers are crucial for single-molecule assays, but noise and drift obscure fundamental behaviors.
- Quantifying the impact of noise reduction techniques in optical tweezers has been challenging.
Purpose of the Study:
- To introduce Allan variance as a method for quantifying noise in optical tweezers.
- To demonstrate its utility in optimizing experimental parameters and assessing noise sources.
Main Methods:
- Application of Allan variance to optical tweezers data.
- Analysis of measurement time, frequency, and detection schemes.
- Quantification of acoustic noise impact.
Main Results:
- Allan variance effectively quantifies noise in optical tweezers.
- Optimal measurement parameters were determined using this method.
- The influence of acoustic noise was successfully quantified.
Conclusions:
- Allan variance is a simple and efficient tool for noise analysis in optical tweezers.
- It enables on-the-fly optimization of experimental parameters.
- This method enhances the reliability of single-molecule assays conducted with optical tweezers.
