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Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+
Published on: June 25, 2018
Variation in amylase activities in radish (Raphanus sativus) cultivars
Masakazu Hara1, Fumio Ito, Tatsuo Asai
1Faculty of Agriculture, Shizuoka University, Suruga-ku, Shizuoka, Japan. masahara@agr.shizuoka.ac.jp
Abstract:
The radish (Raphanus sativus) is a root vegetable of the Brassicaceae family which shows amylolytic activity in the taproot. However, there is little information about differences in these amylolytic activities among radish cultivars. We analyzed the amylase activities and starch contents of 7 kinds of radish cultivars. The Koshin cultivar showed the highest amylase activity, with a level approximately 6 times higher than that of the Sobutori cultivar, which had the lowest. Cultivars with higher amylase activities showed higher starch contents. These results suggest that there are intraspecies variations in amylolytic activities in radishes, and positive correlations between amylase activity and starch content.
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