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Crystallization of Membrane Proteins in Lipidic Mesophases

Crystallization of Membrane Proteins in Lipidic Mesophases

Published on: March 28, 2011

Antisolvent membrane crystallization of pharmaceutical compounds.

Gianluca Di Profio¹, Carmen Stabile, Antonella Caridi

¹Institute on Membrane Technology (ITM-CNR), Via P. Bucci Cubo 17/C, c/o University of Calabria, I-87030 Rende (CS), Italy. g.diprofio@itm.cnr.it

Journal of Pharmaceutical Sciences

|August 6, 2009

Summary

This study introduces a novel membrane crystallization method using vapor phase solvent/antisolvent migration for controlled supersaturation. This technique enhances control over drug crystallization, impacting crystal morphology.

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Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

Published on: November 21, 2010

Area of Science:

Chemical Engineering
Crystallization Science
Materials Science

Background:

Conventional membrane crystallization techniques face limitations in controlling supersaturation.
Precisely controlling supersaturation is crucial for tailoring crystal characteristics.

Purpose of the Study:

To develop and evaluate a modified membrane crystallization technique for enhanced control over supersaturation.
To investigate two distinct configurations for antisolvent membrane crystallization.

Main Methods:

A modified membrane crystallization approach utilizing vapor phase solvent/antisolvent migration.
Two operative configurations: solvent/antisolvent demixing and antisolvent addition.
Testing with paracetamol and glycine to validate the system's efficacy.

Main Results:

Achieved accurate control over solution composition at the crystallization point using both configurations.
Demonstrated that the membrane's porous structure controls vapor phase migration.
Observed significant effects on crystal morphology due to controlled supersaturation.

Conclusions:

The proposed antisolvent membrane crystallization offers a finely controllable supersaturated environment.
This method allows for the generation of crystals with desired characteristics, particularly influencing morphology.
The technique shows promise for industrial applications requiring precise crystallization control.