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Updated: Jun 21, 2026

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
Published on: June 16, 2017
Mechanisms in knockout reactions
D Bazin1, R J Charity, R T de Souza
1National Superconducting Cyclotron Laboratory, Michigan State University, East Lansing, MI 48824, USA. bazin@nscl.msu.edu
Abstract:
We report the first detailed study of the relative importance of the stripping and diffraction mechanisms involved in nucleon knockout reactions, by the use of a coincidence measurement of the residue and fast proton following one-proton knockout reactions. The measurements used the S800 spectrograph in combination with the HiRA detector array at the NSCL. Results for the reactions 9Be(9C,8B+X)Y and 9Be(8B,7Be+X)Y are presented and compared with theoretical predictions for the two reaction mechanisms calculated using the eikonal model. The data show a clear distinction between the stripping and diffraction mechanisms and the measured relative proportions are very well reproduced by the reaction theory. This agreement adds support to the results of knockout reaction analyses and their applications to the spectroscopy of rare isotopes.
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