Protective effect on enamel demineralization of a CPP-ACP paste: an AFM in vitro study
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Updated: Jun 21, 2026

2-Methacryloyloxyethyl Phosphorylcholine Polymer Treatment of Complete Dentures to Inhibit Denture Plaque Deposition
Published on: December 26, 2016
Claudio Poggio1, Marco Lombardini, Alberto Dagna
1Department of Operative Dentistry, University of Pavia, Piazzale Golgi 3, Pavia, Italy. nadine.schlueter@dentist.med.uni-giessen.de
Objectives:
The aim of the present in vitro study was the evaluation of a CPP-ACP paste (Tooth Mousse) on preventing dental erosion produced by a soft drink (Coca Cola), using Atomic Force Microscopy (AFM).
Methods:
Thirty extracted human central incisors free of caries were selected and divided in a treatment and a control half. The treatment halves were divided in three groups-group 1: demineralization with soft drink (4 intervals of 2 min); group 2: demineralization with soft drink (4 intervals of 2 min) plus Tooth Mousse; group 3: intact enamel plus Tooth Mousse. In groups 2 and 3 Tooth Mousse was applied for 3 min at 0, 8, 24 and 36 h. The surface of each specimen was imaged by AFM and R(rms) values were registered.
Results:
Among treatment specimens of groups 1 and 2, a statistically significant difference (P<0.01) in R(rms) values was registered: treatment of the specimens with CPP-ACP paste had a protective effect on enamel demineralization. In group 3 no statistically significant difference was registered between exposed and not exposed halves of the specimens.
Conclusions:
The use of a CPP-ACP paste had a protective effect on enamel demineralization in an in vitro model.

