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Notch and Prospero repress proliferation following cyclin E overexpression in the Drosophila bristle lineage

Françoise Simon1, Pierre Fichelson, Michel Gho

  • 1Université Pierre et Marie Curie-Paris 6, UMR 7622, Paris, France.

Plos Genetics
|August 8, 2009
PubMed

Insights

Post-mitotic cells in adult flies can re-enter the cell cycle when cyclin E is overexpressed. Cell cycle re-entry depends on cell identity, specifically Notch pathway activity and Prospero, regulating differentiation.

Area of Science:

  • Developmental Biology
  • Cell Cycle Regulation
  • Drosophila melanogaster research

Background:

  • Coordinating cell proliferation, arrest, and differentiation is key to understanding normal and pathological development.
  • The adult fly bristle lineage provides a model to study cell cycle control in post-mitotic cells.

Purpose of the Study:

  • To investigate the capacity of post-mitotic cells to re-enter the cell cycle.
  • To determine the factors influencing cell cycle re-entry upon cyclin E overexpression.

Main Methods:

  • Overexpression of cyclin E in the adult fly bristle lineage.
  • Analysis of cell division and differentiation markers.
  • Assessing the role of Notch pathway activity and Prospero.

Main Results:

  • Terminal cells independent of the Notch pathway divided upon cyclin E overexpression.
  • Cellular responsiveness to forced proliferation is modulated by Prospero and Notch pathway activity.
  • Two parallel mechanisms regulate terminal quiescence, differentiation, fate acquisition, and cell cycle progression.

Conclusions:

  • Cellular fate and cell cycle progression are regulated by parallel mechanisms.
  • Notch pathway activity and Prospero are critical regulators of cell cycle re-entry in differentiating cells.

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