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Related Experiment Video

Updated: Jun 21, 2026

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer
12:23

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

Published on: August 2, 2018

Phosphorus-based absolutely quantified standard peptides for quantitative proteomics.

Nico Zinn1, Bettina Hahn, Rüdiger Pipkorn

  • 1Molecular Structure Analysis, German Cancer Research Center, Heidelberg, Germany.

Journal of Proteome Research
|August 12, 2009
PubMed
Summary
This summary is machine-generated.

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A new method produces precisely quantified peptide standards using phosphorus-based absolutely quantified standard (PASTA) peptides. This technique ensures accuracy for peptide quantification in complex biological samples.

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Mass Spectrometry

Background:

  • Accurate quantification of peptide standards is crucial for proteomics and biomarker discovery.
  • Existing methods for peptide standard quantification can be complex and lack absolute accuracy.

Purpose of the Study:

  • To develop an innovative method for producing absolutely quantified peptide standards.
  • To establish phosphorus-based absolutely quantified standard (PASTA) peptides for accurate quantification.

Main Methods:

  • Utilized a hybrid LC-(ICP+ESI)-MS system for calibrating synthetic phosphopeptides.
  • Employed ICP-MS detection of 31P for quantification and ESI-MS for identification.
  • Developed dephosphorylation strategies (enzymatic/chemical) and stable isotope labeling for peptide standard generation.

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Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae
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Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae

Published on: October 12, 2009

Related Experiment Videos

Last Updated: Jun 21, 2026

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer
12:23

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

Published on: August 2, 2018

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae
15:41

Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae

Published on: October 12, 2009

Main Results:

  • Achieved absolute concentrations for phosphopeptide standards with accuracy better than 10%.
  • Demonstrated the production of cognate peptide standards from phosphopeptides via dephosphorylation.
  • Successfully employed stable isotope-labeled PASTA peptides as internal standards for LC-ESI-MS analysis.

Conclusions:

  • The PASTA peptide method provides a robust approach for absolute quantification of peptide standards.
  • This technique enables accurate measurement of peptides, including post-translational modifications.
  • The developed method facilitates precise quantification of proteins like MAP kinase p38alpha.