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Related Concept Videos

Bone Disorders01:29

Bone Disorders

Aging and its effect on bone remodeling is the most common cause of bone disorders. In young and healthy people, bone deposition and resorption happen at an equal rate to maintain optimal bone health.
Bone deposition is also affected by the levels of sex hormones like estrogen and testosterone that promote osteoblast activity and bone matrix synthesis. When the level of these hormones decreases due to aging, it causes a reduction in bone deposition. As a result, bone resorption by osteoclasts...
The Effect of Aging on Tissues01:19

The Effect of Aging on Tissues

Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...
Cross-bridge Cycle01:26

Cross-bridge Cycle

As muscle contracts, the overlap between the thin and thick filaments increases, decreasing the length of the sarcomere—the contractile unit of the muscle—using energy in the form of ATP. At the molecular level, this is a cyclic, multistep process that involves binding and hydrolysis of ATP, and movement of actin by myosin.
Changes in the Appendicular Skeleton with Age01:09

Changes in the Appendicular Skeleton with Age

The upper and lower limb initially develops as a small bulge called a limb bud, which appears on the lateral side of the early embryo. The upper limb bud appears near the end of the fourth week of development, with the lower limb bud appearing shortly after.
Initially, the limb buds consist of a core of mesenchyme covered by a layer of ectoderm. The ectoderm at the end of the limb bud thickens to form a narrow crest called the apical ectodermal ridge. This ridge stimulates the underlying...
Disorders of the Skeletal Muscle01:28

Disorders of the Skeletal Muscle

The clinical conditions affecting the skeletal muscle tissue are broadly categorized as musculoskeletal and neuromuscular disorders.
Musculoskeletal disorders
Musculoskeletal disorders involve injuries and conditions affecting the skeletal muscles and associated connective tissues. These disorders can arise from acute biomechanical stresses or chronic overuse and can occur across different age groups. Common injuries include sprains, fractures, and muscular strains, often resulting from...
Mitochondria01:37

Mitochondria

Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...

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Related Experiment Video

Updated: Jun 21, 2026

Ex Vivo Assessment of Contractility, Fatigability and Alternans in Isolated Skeletal Muscles
14:02

Ex Vivo Assessment of Contractility, Fatigability and Alternans in Isolated Skeletal Muscles

Published on: November 1, 2012

Increased CaVbeta1A expression with aging contributes to skeletal muscle weakness.

Jackson R Taylor1, Zhenlin Zheng, Zhong-Min Wang

  • 1Department of Internal Medicine-Gerontology, Wake Forest University School of Medicine, 1 Medical Center Boulvard, Winston Salem, NC 27157, USA.

Aging Cell
|August 12, 2009
PubMed
Summary

Overexpression of beta-1a subunits (CaVbeta1a) in aging skeletal muscle leads to reduced specific force by decreasing CaV1.1 expression. This suggests CaVbeta1a contributes to age-related muscle weakness.

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Human Vastus Lateralis Skeletal Muscle Biopsy Using the Weil-Blakesley Conchotome
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Human Vastus Lateralis Skeletal Muscle Biopsy Using the Weil-Blakesley Conchotome

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Related Experiment Videos

Last Updated: Jun 21, 2026

Ex Vivo Assessment of Contractility, Fatigability and Alternans in Isolated Skeletal Muscles
14:02

Ex Vivo Assessment of Contractility, Fatigability and Alternans in Isolated Skeletal Muscles

Published on: November 1, 2012

Human Vastus Lateralis Skeletal Muscle Biopsy Using the Weil-Blakesley Conchotome
07:16

Human Vastus Lateralis Skeletal Muscle Biopsy Using the Weil-Blakesley Conchotome

Published on: March 4, 2016

Area of Science:

  • Muscle physiology
  • Skeletal muscle aging
  • Excitation-contraction coupling

Background:

  • Excitation-contraction (E-C) coupling is vital for muscle function.
  • Age-related muscle weakness is linked to E-C uncoupling.
  • CaV1.1 and CaVbeta1a subunits are essential for E-C coupling.

Purpose of the Study:

  • Investigate the role of CaVbeta1a in aging skeletal muscle.
  • Examine the relationship between CaVbeta1a and CaV1.1 expression.
  • Determine the impact of CaVbeta1a on specific muscle force.

Main Methods:

  • Western blot analysis of CaVbeta1a expression in aging mice.
  • In vivo electroporation of CaVbeta1a-YFP in young mice.
  • Whole-cell patch-clamp to measure charge movement.
  • siRNA interference to modulate CaVbeta1a levels.

Main Results:

  • CaVbeta1a expression significantly increases with age in FVB mice.
  • Overexpression of CaVbeta1a in young muscle reduces CaV1.1 expression.
  • Increased CaVbeta1a correlates with decreased charge movement and specific force.
  • CaVbeta1a knockdown in young muscle reduces charge movement; in old muscle, it restores it.

Conclusions:

  • CaVbeta1a acts as a dual regulator of CaV1.1 expression.
  • Endogenous CaVbeta1a overexpression in aged muscle contributes to loss of specific force.
  • CaVbeta1a is a potential therapeutic target for age-related muscle decline.