Localization of virus and antibody response in mice infected persistently with MHV-JHM

Suckling mice infected intranasally with MHV-JHM and nursed by immunized dams develop a late onset demyelinating encephalomyelitis. Analysis by in situ hybridization revealed that MHV-JHM entered the central nervous system (CNS) via the olfactory and trigeminal nerves and spread over the next two weeks to the spinal cord, prior to amplification at this site. Serial measurements of neutralizing antibody titers showed that the late onset disease developed in some mice at levels of antibody which protected mice from the fatal, acute encephalitis, supporting the notion that cell-mediated and not humoral immunity is important in protecting mice from MHV-JHM persistence.