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Enzyme-release capillary as a facile enzymatic biosensing part for a capillary-assembled microchip
Terence G Henares1, Eiji Maekawa, Fumito Okubo
1Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, Sakai, Japan.
Abstract:
A simple capillary enzymatic biosensor was developed. This was prepared by simply coating a dissolvable membrane containing enzyme/s on the inner wall of a square glass capillary. An easy measurement was carried out by capillary force sample introduction with concurrent enzyme release and a reaction with a certain substrate. Enzyme-release capillary (ERC) biosensors showed long-term storage stability of at least two weeks for a beta-galactoside derivative and glucose. Moreover, this could be integrated on a capillary-assembled microchip (CAs-CHIP) to broaden its multiple analyte sensing potential for clinical diagnostic applications.
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