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Updated: Jun 21, 2026

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes
Published on: January 16, 2016
Ribozymes: analytical solution of the one-substrate, two-intermediate reversible scheme for enzyme reactions
Paolo Toti1, Ludovico Sbordone, Carolina Sbordone
1Unità di Biochimica, Dipartimento di Biologia, Università di Pisa, 56127 Pisa, Italy.
Abstract:
The paper presents a kinetic analysis of a reversible enzymatic reaction Sright arrow over left arrowP involving two intermediate compounds under the condition [E](0) >> [S](0) + [P](0). For the case of mono-exponential behavior, we derive an equation for k (obs) as a function of [E](0), which emphasizes the pitfalls of oversimplifying kinetic schemes (such as the Michaelis-Menten model) for ribozyme studies. This novel apparent rate constant, which has been arrived at through mechanistic considerations, is analyzed, and the characteristic parameters obtained. The equation, which seems to fit experimental data better than conventional approximations, is used to analyze a single turnover study on an ADC1 ribozyme drawn from hepatitis delta virus RNA. The microscopic kinetic constants for such enzyme are evaluated and its mono-exponential behavior verified.
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