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Thrombin decreases platelet cyclic AMP in the absence of prostaglandin synthesis
Cyclic AMP first decreased in wahed platelets after the addition of 0.1 IU thrombin/ml and then increased progressively. Platelets pre-incubated with acetylsalicylic acid showed only a decrease of cyclic AMP upon addition of thrombin. Furthermore, two patients with defective prostaglandin synthesis had always reduced cyclic AMP whether their platelets were pre-incubated with acetylsalicylic acid or not. Thus, if prostaglandin synthesis is inhibited the level of cyclic AMP decreased and did not increase with time.
Cyclic AMP first decreased in wahed platelets after the addition of 0.1 IU thrombin/ml and then increased progressively. Platelets pre-incubated with acetylsalicylic acid showed only a decrease of cyclic AMP upon addition of thrombin. Furthermore, two patients with defective prostaglandin synthesis had always reduced cyclic AMP whether their platelets were pre-incubated with acetylsalicylic acid or not. Thus, if prostaglandin synthesis is inhibited the level of cyclic AMP decreased and did not increase with time.