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Updated: Jun 21, 2026

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Published on: April 6, 2016

Immobilized monolithic enzyme reactors for application in proteomics and pharmaceutics.

Jens Spross¹, Andrea Sinz

¹Department of Pharmaceutical Chemistry & Bioanalytics, Institute of Pharmacy, Martin-Luther-Universität Halle-Wittenberg, Wolfgang-Langenbeck-Strasse 4, 06120, Halle, Saale, Germany.

Analytical and Bioanalytical Chemistry

|August 12, 2009

Summary

Monolithic supports offer superior chromatographic performance for bioreactors and affinity media compared to traditional particle-based systems. Their ease of fabrication and durability make them ideal for automated analytical applications.

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Area of Science:

Materials Science
Analytical Chemistry
Biotechnology

Background:

Monolithic supports are increasingly utilized across diverse scientific applications.
Conventional particle-based systems face limitations in certain chromatographic applications.

Purpose of the Study:

To evaluate the chromatographic performance of monolithic supports in bioreactors and affinity media.
To compare monolithic media with conventional particle-based systems.
To highlight the advantages of monolithic supports for analytical applications.

Main Methods:

Fabrication and modification of monolithic columns.
Chromatographic performance evaluation.
Application in bioreactors and affinity media.

Main Results:

Monolithic media demonstrated superior chromatographic performance over particle-based systems.
Monolithic columns exhibit ease of fabrication and modification.
Long operational lifetimes and suitability for automated systems were observed.

Conclusions:

Monolithic supports provide enhanced chromatographic capabilities for bioreactors and affinity media.
The characteristics of monolithic columns make them highly attractive for advanced analytical systems.
Widespread adoption of monolithic supports is anticipated due to their performance and versatility.