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Updated: Jun 21, 2026

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
Published on: July 6, 2016
Highly efficient synthesis of DNA-binding hairpin polyamides via the use of a new triphosgene coupling strategy
Wu Su1, Stephen J Gray, Ruggero Dondi
1Department of Chemistry, University of Leicester, University Road, Leicester, UK.
Abstract:
A facile and highly efficient solid phase synthesis method is reported for the preparation of hairpin DNA-binding polyamides using the cost-effective triphosgene (BTC) activating agent. Difficult polyamide sequences were prepared from N-methylimidazole (Im) and N-methylpyrrole (Py) building blocks with high stepwise yields (>98%) using Boc chemistry. The versatility of the triphosgene coupling approach was also demonstrated for the first time on aryl hydrazine resins to afford biomedically relevant tail-truncated polyamides in excellent isolated yields.
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