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Identification of target genes of transcription factor activator protein 2 gamma in breast cancer cells

He Ailan1, Xiao Xiangwen, Ren Daolong

  • 1Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China, PR China. pdheailan@126.com

BMC Cancer
|August 13, 2009
PubMed
Abstract

Insights

Activator protein 2 gamma (AP-2gamma) coordinates genes in breast cancer. This transcription factor directly regulates ErbB2, CDH2, HPSE, and IGSF11, offering potential therapeutic targets for cancer treatment.

Area of Science:

  • Molecular Biology
  • Genetics
  • Oncology

Background:

  • Activator protein 2 gamma (AP-2gamma), a transcription factor, is implicated in human neoplasia.
  • AP-2gamma is overexpressed in most breast cancers, exhibiting a dual role in tumorigenesis.

Purpose of the Study:

  • To identify gene targets mediating AP-2gamma's effects in breast cancer.
  • To understand the regulatory network controlled by AP-2gamma.

Main Methods:

  • Chromatin immunoprecipitation (ChIP) was used to identify AP-2gamma binding sites.
  • A combination of sequence analysis, reporter gene assays, qPCR, EMSA, and immunoblot analysis confirmed target genes.

Main Results:

  • Twenty novel DNA fragments near potential AP-2gamma targets were identified.
  • Four key genes involved in carcinogenesis were confirmed as AP-2gamma targets: ErbB2, CDH2, HPSE, and IGSF11.
  • AP-2gamma directly regulates these four genes, as evidenced by expression changes in breast cancer cells.

Conclusions:

  • AP-2gamma coordinates a network of genes involved in breast cancer development.
  • These identified AP-2gamma target genes represent potential therapeutic targets for breast cancer treatment.

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