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Biology under construction: in vitro reconstitution of cellular function
Allen P Liu1, Daniel A Fletcher
1Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
Nature Reviews. Molecular Cell Biology
|August 13, 2009
Summary
Scientists are improving at reconstituting biological processes from molecules. This bottom-up approach advances basic and applied sciences by uncovering fundamental cellular assembly mechanisms.
Area of Science:
- Cell biology
- Biochemistry
- Systems biology
Background:
- Studying functional organization in biology is challenging.
- Reconstitution of biological processes from component molecules is a powerful but difficult approach.
- Advances in biochemical and cell biological techniques are enabling new research.
Purpose of the Study:
- To explore the potential of bottom-up strategies in biology.
- To understand the basic processes guiding cellular assembly.
- To advance both basic and applied biological sciences.
Main Methods:
- Utilizing a convergence of biochemical and cell biological advances.
- Employing new experimental and computational tools.
- Applying a bottom-up strategy for biological process reconstitution.
Main Results:
- The study highlights improved capabilities in reconstituting biological processes.
- Complex biological processes are becoming increasingly amenable to bottom-up reconstitution.
- The approach is uncovering fundamental mechanisms of cellular assembly.
Conclusions:
- The bottom-up reconstitution strategy is a promising approach for biological research.
- This strategy will uncover basic processes that guide cellular assembly.
- Advancements in this field will benefit both basic and applied biological sciences.
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