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Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.
Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...

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Related Experiment Video

Updated: Jun 21, 2026

Experimental Autoimmune Uveitis: An Intraocular Inflammatory Mouse Model
07:40

Experimental Autoimmune Uveitis: An Intraocular Inflammatory Mouse Model

Published on: January 12, 2022

EAE: an immunologist's magic eye.

Gurumoorthy Krishnamoorthy1, Hartmut Wekerle

  • 1Department of Neuroimmunology, Max Planck Institute of Neurobiology, Martinsried, Germany. guru@neuro.mpg.de

European Journal of Immunology
|August 13, 2009
PubMed
Summary
This summary is machine-generated.

Experimental autoimmune encephalomyelitis (EAE) exhibits diverse variants. Underlying these complexities are fundamental patterns of autoimmunity and self-tolerance, revealing crucial insights into immune responses.

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Last Updated: Jun 21, 2026

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Visualizing Impairment of the Endothelial and Glial Barriers of the Neurovascular Unit during Experimental Autoimmune Encephalomyelitis In Vivo
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Visualizing Impairment of the Endothelial and Glial Barriers of the Neurovascular Unit during Experimental Autoimmune Encephalomyelitis In Vivo

Published on: March 26, 2019

Area of Science:

  • Immunology
  • Neuroscience
  • Autoimmunity

Background:

  • Experimental autoimmune encephalomyelitis (EAE) is a complex disease model.
  • EAE presents with diverse clinical, immunological, and structural variations.
  • Understanding these variants is key to deciphering immune responses.

Purpose of the Study:

  • To identify underlying patterns within the complexity of EAE variants.
  • To relate EAE variations to fundamental principles of autoimmunity and self-tolerance.
  • To provide a new perspective for immunologists studying EAE.

Main Methods:

  • Analysis of clinical, immunological, and structural data from EAE models.
  • Pattern recognition within diverse EAE presentations.
  • Comparative analysis of EAE variants.

Main Results:

  • EAE variants, despite their complexity, exhibit discernible patterns.
  • These patterns reflect fundamental aspects of immune response, including autoimmunity and self-tolerance.
  • EAE can be viewed as an autostereogram, revealing hidden structures.

Conclusions:

  • The apparent chaos of EAE variants conceals fundamental immunological principles.
  • Recognizing these patterns offers a powerful tool for understanding autoimmunity.
  • EAE serves as a valuable model for studying self-tolerance and immune dysregulation.