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Updated: Jun 21, 2026
A Time Differential Staining Technique Coupled with Full Bilateral Gill Denervation to Study Ionocytes in Fish
Published on: March 19, 2015
Effect of histological processing and methacrylate sectioning on the area of gill tissue in teleost
A L Cruz1, M N Fernandes, S F Perry
1Departamento de Ciências Fisiológicas, Universidade Federal de São Carlos, São Carlos, SP, Brazil, 13565-905.
Abstract:
Deformation of biological tissues may occur during histological processing and results in loss of accuracy when quantitative information about cells, tissues and organs is necessary. In this study, the gill tissue from armored catfish (Pterygoplichthys anisitsi) was quantified in each step of processing using the stereological principles. During processing for glycol methacrylate embedding, gill tissue from shrinks significantly but regains its original dimensions after sectioning.
