Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Electron Transport Chain01:30

The Electron Transport Chain

The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q in...
Oxygen Requirements and Growth Patterns01:29

Oxygen Requirements and Growth Patterns

Microorganisms exhibit diverse oxygen requirements and growth patterns driven by their metabolic strategies and environmental adaptations. Oxygen, while essential for many organisms, can also be toxic under certain conditions, shaping how microorganisms grow and survive.Oxygen Requirements of MicroorganismsMicroorganisms are classified based on their ability to use or tolerate oxygen:● Obligate aerobes like Mycobacterium tuberculosis need oxygen for energy production, as it serves as the...
Electron Transport Chain: Complex III and IV01:43

Electron Transport Chain: Complex III and IV

During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quinolone tolerance in <i>Escherichia coli</i> due to defects in the adenosine ribonucleotides <i>de novo</i> biosynthesis pathway.

mLife·2026
Same author

CRISPR-Cas-associated SCCmec Variants in Methicillin-resistant Staphylococcus aureus Evade Rapid Diagnostic Detection.

The Journal of infectious diseases·2025
Same author

Prophage-encoded methyltransferase drives adaptation of community-acquired methicillin-resistant Staphylococcus aureus.

The Journal of clinical investigation·2025
Same author

Antibiotic-persistent bacterial cells exhibiting low-level ROS are eradicated by ROS-independent membrane disruption.

mBio·2025
Same author

Broad-spectrum tolerance to disinfectant-mediated bacterial killing due to mutation of the PheS aminoacyl tRNA synthetase.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Antibiotic tolerance due to restriction of cAMP-Crp regulation by mannitol, a non-glucose-family PTS carbon source.

mSphere·2024

Related Experiment Video

Updated: Jun 21, 2026

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K
06:45

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K

Published on: January 11, 2019

Superoxide protects Escherichia coli from bleomycin mediated lethality.

Richard M Burger1, Karl Drlica

  • 1Public Health Research Institute, New Jersey Medical School, UMDNJ, Newark, 07103, USA. burger_lic@yahoo.com

Journal of Inorganic Biochemistry
|August 15, 2009
PubMed
Summary

Superoxide interferes with bleomycin

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Background:

  • Reactive oxygen species, including superoxide and hydroxyl radical, are implicated in antimicrobial-induced cell death.
  • The precise mechanisms by which these reactive species influence bacterial responses to antibiotics are not fully understood.

Purpose of the Study:

  • To investigate the role of superoxide in bleomycin-induced cytotoxicity in Escherichia coli.
  • To elucidate the mechanisms by which superoxide affects bleomycin lethality, independent of known oxidative stress defense pathways.

Main Methods:

  • Utilized genetic manipulation in Escherichia coli, including strains deficient in superoxide dismutase and varying recA and soxRS regulon states.
  • Employed plumbagin, a superoxide generator, to assess its effect on bleomycin lethality.

More Related Videos

Studying Oxidative Stress Caused by the Mitis Group Streptococci in Caenorhabditis elegans
09:36

Studying Oxidative Stress Caused by the Mitis Group Streptococci in Caenorhabditis elegans

Published on: March 23, 2019

Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5&#8242;-Phosphate
08:25

Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate

Published on: April 6, 2022

Related Experiment Videos

Last Updated: Jun 21, 2026

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K
06:45

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K

Published on: January 11, 2019

Studying Oxidative Stress Caused by the Mitis Group Streptococci in Caenorhabditis elegans
09:36

Studying Oxidative Stress Caused by the Mitis Group Streptococci in Caenorhabditis elegans

Published on: March 23, 2019

Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5&#8242;-Phosphate
08:25

Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate

Published on: April 6, 2022

  • Compared bleomycin cytotoxicity in different genetic backgrounds under varying oxidative conditions.

    • Main Results:

    • Genetic deficiency of superoxide dismutase ameliorated bleomycin lethality.
    • Supplementation with plumbagin significantly rescued Escherichia coli from bleomycin-induced cell death.
    • This rescue effect by plumbagin was observed irrespective of the recA gene status or the activity of the soxRS regulon.

    Conclusions:

    • Superoxide plays a critical role in modulating bleomycin cytotoxicity in Escherichia coli.
    • The protective effect of superoxide against bleomycin is mediated through pathways distinct from canonical oxidative damage defense mechanisms.
    • Findings challenge existing models of oxidative stress response in bacterial antibiotic resistance.