Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antibiotic Selection00:57

Antibiotic Selection

Overview
Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
Antibody Actions01:26

Antibody Actions

Antibodies, or immunoglobulins, are critical players in the immune system's arsenal against invading pathogens. Produced by B cells and plasma cells, their primary role is to detect and bind to specific antigens, molecules found on the surface of pathogens like bacteria or viruses. Beyond antigen recognition, antibodies perform several vital functions that contribute to immune defense.
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrative networks regulating tomato fruit locule number through shoot apical meristem size control: bridging genetic, phytohormonal and environmental factors.

Planta·2026
Same author

Modular microfluidic probe for addressable fluidic landscapes.

Lab on a chip·2026
Same author

Modulation of antioxidant systems and photosynthetic machinery by foliar-applied ZnO nanoparticles in cadmium-stressed mung bean (Vigna radiata L.).

BMC plant biology·2026
Same author

Feature tracking-based motility analysis of excised intestinal tissue using video recordings.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2025
Same author

Spheromatrix: a paper-based platform for scalable 3D tumor model generation, cryopreservation, and high-throughput drug assessment.

Microsystems & nanoengineering·2025
Same author

Detailed microCT imaging protocol for ex vivo rat stomachs with comparative analysis.

Scientific reports·2025

Related Experiment Video

Updated: Jun 21, 2026

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
10:17

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

Antibody selection for immobilizing living bacteria.

Zhiyong Suo1, Xinghong Yang, Recep Avci

  • 1Department of Physics, Montana State University, Bozeman, Montana 59717, USA.

Analytical Chemistry
|August 18, 2009
PubMed
Summary

Antibodies targeting CFA/I fimbriae effectively immobilized Salmonella Typhimurium. Targeting surface antigens extending from the cell wall, like CFA/I fimbriae, is crucial for efficient bacterial immunoimmobilization.

More Related Videos

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
12:55

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments
12:28

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments

Published on: October 15, 2016

Related Experiment Videos

Last Updated: Jun 21, 2026

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
10:17

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
12:55

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments
12:28

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments

Published on: October 15, 2016

Area of Science:

  • Microbiology
  • Immunology
  • Bacterial Pathogenesis

Background:

  • Salmonella enterica Serovar Typhimurium is a significant human pathogen.
  • Effective methods for bacterial capture and immobilization are essential for diagnostics and research.
  • Bacterial surface antigens offer potential targets for antibody-mediated immobilization.

Purpose of the Study:

  • To comparatively evaluate the efficacy of seven antibodies in immobilizing Salmonella Typhimurium.
  • To identify optimal bacterial surface antigens for antibody-based immunoimmobilization.
  • To determine the viability and longevity of immobilized bacteria.

Main Methods:

  • Comparative analysis of antibody efficacy against S. Typhimurium surface antigens: CFA/I fimbriae, flagella, lipopolysaccharides (LPS), and capsular F1 antigen.
  • Assessment of immobilization efficiency based on antibody binding to specific antigens.
  • Investigation of flagella paralysis impact on antiflagellin antibody efficacy.
  • Evaluation of bacterial viability and microarray functionality over time.

Main Results:

  • Antibodies targeting CFA/I fimbriae demonstrated the highest efficacy in S. Typhimurium immobilization.
  • Antiflagellin antibody efficacy was enhanced by paralyzing flagellar motion.
  • Antibodies against O-antigen polysaccharides showed moderate immobilization; others targeting LPS were less effective.
  • Antibodies against the F1 antigen were ineffective, likely due to antigen detachment.
  • Immobilized bacteria remained viable for at least two weeks.

Conclusions:

  • Antibody-mediated bacterial immunoimmobilization is most effective when targeting surface antigens that are both exposed and firmly attached to the bacterial cell wall.
  • CFA/I fimbriae represent a highly suitable target antigen for S. Typhimurium immobilization.
  • Immobilized S. Typhimurium microarrays maintain viability, offering potential for stable biosensor applications.