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Methyl-binding DNA capture Sequencing for Patient Tissues

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

Sensitive digital quantification of DNA methylation in clinical samples.

Meng Li¹, Wei-Dong Chen, Nickolas Papadopoulos

¹The Ludwig Center for Cancer Genetics and Therapeutics, Howard Hughes Medical Institute and Sidney Kimmel Cancer Center at the Johns Hopkins Medical Institutions, Baltimore, Maryland, USA.

Nature Biotechnology

|August 18, 2009

Summary

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We developed methyl-BEAMing, a digital technology for precise quantification of methylated DNA molecules. This method shows promise for early cancer detection using biomarkers in plasma and stool samples.

Area of Science:

Molecular Biology
Epigenetics
Biomarker Discovery

Background:

Abnormally methylated genes are crucial in cancer research and biomarker development.
Accurate quantification of methylated molecules is essential for these applications.

Purpose of the Study:

To develop a technology for absolute quantification of methylated DNA molecules.
To assess the utility of methyl-BEAMing for cancer biomarker detection.

Main Methods:

Developed methyl-BEAMing technology for absolute quantification of methylated DNA.
Utilized flow cytometry and next-generation sequencing for analysis.
Applied the technology to plasma and fecal DNA samples.

Main Results:

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Last Updated: Jun 20, 2026

Methyl-binding DNA capture Sequencing for Patient Tissues

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Published on: February 24, 2015

Enumerated as few as one methylated molecule among 5,000 unmethylated molecules.
Detected 59% of cancer cases using methylated vimentin in plasma.
Detected 41% of cancers and 45% of advanced adenomas in stool samples.
Achieved fourfold higher sensitivity than CEA assay for early-stage colorectal cancer detection.

Conclusions:

Methyl-BEAMing enables precise digital quantification of rare epigenetic events.
This technology has significant potential for cancer diagnostics and prognostics.
Applicable to preclinical biomarker assessment and epigenetic research.