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Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
Improved ethanol tolerance in Escherichia coli by changing the cellular fatty acids composition through genetic
Lian Hua Luo1, Pil-Soo Seo, Jeong-Woo Seo
1Molecular Bioprocess Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, 580-185, Jeonbuk, South Korea.
Abstract:
To investigate the effect of cellular fatty acids composition on ethanol tolerance in Escherichia coli, we overexpressed either des, encoding fatty acid desaturase from Bacillus subtilis, or fabA, encoding beta-hydroxydecanoyl thio-ester dehydrase from E. coli, or both genes together, into E. coli. Recombinant E. coli harboring fabA had elevated tolerance against ethanol compared to wild type strain. In contrast, des decreased resistance to ethanol. Co-expression of both genes together complemented ethanol tolerance of E. coli. This result indicates how to engineer bacterial strains to be resistant to higher concentrations of ethanol.
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