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Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then passed on to...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Mass Spectrometers01:16

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...

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Updated: Jun 20, 2026

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
05:47

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

Published on: February 19, 2020

Ambient imaging mass spectrometry by electrospray ionization using solid needle as sampling probe.

Lee Chuin Chen1, Kentaro Yoshimura, Zhan Yu

  • 1Clean Energy Research Center, University of Yamanashi, Takeda 4-3-11, Kofu, 400-8511, Japan. leechuin.yamanashi@gmail.com

Journal of Mass Spectrometry : JMS
|August 18, 2009
PubMed
Summary
This summary is machine-generated.

Probe electrospray ionization (PESI) enables direct ambient imaging mass spectrometry of mouse brain sections. This technique successfully mapped lipids with 60 micrometer resolution, advancing in-situ biological tissue analysis.

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Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

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Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions
15:00

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

Published on: June 14, 2016

Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Mass Spectrometry Imaging

Background:

  • Traditional electrospray ionization (ESI) requires liquid solutions, limiting its direct application in ambient imaging mass spectrometry.
  • Probe electrospray ionization (PESI) offers direct sampling from wet samples using a solid needle probe, overcoming ESI limitations.
  • Biological tissues, rich in water, present unique challenges and opportunities for direct ambient mass spectrometry.

Purpose of the Study:

  • To demonstrate the feasibility of ambient imaging mass spectrometry on biological tissue sections using PESI.
  • To develop and validate a method for high-resolution lipid mapping in mouse brain tissue.
  • To investigate the utility of PESI coupled with an auxiliary heated capillary sprayer for enhanced sample ionization and analysis.

Main Methods:

  • Utilized probe electrospray ionization (PESI) with a solid needle probe for direct sampling of paraformaldehyde-fixed mouse brain sections.
  • Incorporated an auxiliary heated capillary sprayer to generate solvent vapor, aiding sample re-dissolution and needle cleaning.
  • Employed automated spatial analysis with controlled sampling depth and raster scanning to achieve 60 micrometer lateral resolution.

Main Results:

  • Successfully generated ambient mass spectrometric images of a mouse brain section.
  • Detected and mapped phospholipids and galactosylceramides in positive ion mode with high spatial resolution.
  • Demonstrated the capability of PESI for in-situ lipid profiling of biological tissues.

Conclusions:

  • PESI, when coupled with an auxiliary heated capillary sprayer, is a viable technique for ambient imaging mass spectrometry of biological tissues.
  • The method provides high-resolution spatial distribution maps of key lipids within the mouse brain.
  • This approach offers a promising alternative for direct, in-situ analysis of complex biological samples.