Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SHY2 as a node in the regulation of root meristem development by auxin, brassinosteroids, and cytokinin.

Journal of integrative plant biology·2020
Same author

Dynamic tailoring of an optical skyrmion lattice in surface plasmon polaritons.

Optics express·2020
Same author

High-Efficiency, Broadband, Near Diffraction-Limited, Dielectric Metalens in Ultraviolet Spectrum.

Nanomaterials (Basel, Switzerland)·2020
Same author

Experimental Examination of Electrical Characteristics for Portland Cement Mortar Frost Damage Evaluation.

Materials (Basel, Switzerland)·2020
Same author

Synthesis of Imidazole-Based Medicinal Molecules Utilizing the van Leusen Imidazole Synthesis.

Pharmaceuticals (Basel, Switzerland)·2020
Same author

Mutagenesis for Improvement of Activity and Stability of Prolyl Aminopeptidase from Aspergillus oryzae.

Applied biochemistry and biotechnology·2020

Related Experiment Video

Updated: Jun 20, 2026

Ultrahigh Density Array of Vertically Aligned Small-molecular Organic Nanowires on Arbitrary Substrates
08:07

Ultrahigh Density Array of Vertically Aligned Small-molecular Organic Nanowires on Arbitrary Substrates

Published on: June 18, 2013

Fabrication of protein nanotubes using template-assisted electrostatic layer-by-layer methods.

Shelley A Dougherty1, Dawei Zhang, Jianyu Liang

  • 1Department of Mechanical Engineering, Worcester Polytechnic Institute, 100 Institute Rd, Worcester, Massachusetts 01609, USA.

Langmuir : the ACS Journal of Surfaces and Colloids
|August 19, 2009
PubMed
Summary

Researchers developed a method for fabricating single-component protein nanotubes. This work advances the creation of entirely protein nanostructures for biomedical applications.

More Related Videos

Fabrication, Densification, and Replica Molding of 3D Carbon Nanotube Microstructures
09:23

Fabrication, Densification, and Replica Molding of 3D Carbon Nanotube Microstructures

Published on: July 2, 2012

Template Directed Synthesis of Plasmonic Gold Nanotubes with Tunable IR Absorbance
13:37

Template Directed Synthesis of Plasmonic Gold Nanotubes with Tunable IR Absorbance

Published on: April 1, 2013

Related Experiment Videos

Last Updated: Jun 20, 2026

Ultrahigh Density Array of Vertically Aligned Small-molecular Organic Nanowires on Arbitrary Substrates
08:07

Ultrahigh Density Array of Vertically Aligned Small-molecular Organic Nanowires on Arbitrary Substrates

Published on: June 18, 2013

Fabrication, Densification, and Replica Molding of 3D Carbon Nanotube Microstructures
09:23

Fabrication, Densification, and Replica Molding of 3D Carbon Nanotube Microstructures

Published on: July 2, 2012

Template Directed Synthesis of Plasmonic Gold Nanotubes with Tunable IR Absorbance
13:37

Template Directed Synthesis of Plasmonic Gold Nanotubes with Tunable IR Absorbance

Published on: April 1, 2013

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Protein Engineering

Background:

  • One-dimensional protein nanostructures hold significant promise for biomedical applications.
  • Current fabrication methods often result in composite materials, with few achieving single-component protein nanostructures.
  • Developing methods for entirely protein-based nanostructures is a key goal.

Purpose of the Study:

  • To develop a fabrication method for single-component protein nanotubes.
  • To investigate template pretreatment methods for enhancing protein layer adhesion.
  • To address limitations in existing layer-by-layer assembly techniques for protein nanostructures.

Main Methods:

  • Utilized a layer-by-layer electrostatic assembly method adapted from existing literature.
  • Investigated two distinct template pretreatment protocols to improve protein-template interactions.
  • Modified the established fabrication protocol based on critical findings during the study.

Main Results:

  • Identified a significant flaw in previously published layer-by-layer assembly methods for protein nanostructures.
  • Successfully fabricated glucose oxidase/avidin nanostructures using a modified electrostatic layer-by-layer assembly approach.
  • Demonstrated the efficacy of one specific template pretreatment method.

Conclusions:

  • The study successfully fabricated single-component protein nanostructures (glucose oxidase/avidin).
  • A critical limitation in prior methods was identified and addressed through protocol modification.
  • The findings contribute to the advancement of fabricating entirely protein-based nanostructures for potential biomedical uses.