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Microscopy Techniques for Interpreting Fungal Colonization in Mycoheterotrophic Plants Tissues and Symbiotic Germination of Seeds
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Scoring of Mohs tissue in one-piece processing to prevent tissue crumbling or detachment from the embedding medium

Sharron Ladd1, Basil S Cherpelis

  • 1Department of Dermatology and Cutaneous Surgery, University of South Florida, Tampa, Florida 33612, USA. sladd@health.usf.edu

Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [Et Al.]
|August 19, 2009
PubMed
Summary

No abstract available in PubMed .

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Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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