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Published on: January 6, 2018
Structural characterization of edematous corneas by forward and backward second harmonic generation imaging
Chiu-Mei Hsueh1, Wen Lo, Wei-Liang Chen
1Department of Physics, National Taiwan University, Taipei, Taiwan.
Biophysical Journal
|August 19, 2009
Summary
Second Harmonic Generation (SHG) microscopy effectively images corneal edema's structural changes. This technique reveals microscopic alterations in the posterior cornea, aiding in edema detection and diagnosis.
Area of Science:
- Ophthalmology
- Biophysics
- Microscopy
Background:
- Corneal edema involves structural changes impacting vision.
- Understanding these alterations is crucial for diagnosis and treatment.
- Current imaging methods may lack microscopic detail.
Purpose of the Study:
- To image and quantify structural changes in corneal edema using SHG microscopy.
- To characterize morphological differences between normal and edematous bovine corneas.
- To evaluate SHG imaging for detecting and diagnosing corneal edema.
Main Methods:
- Utilized bovine cornea as an experimental model.
- Employed second harmonic generation (SHG) microscopy.
- Simultaneously collected forward and backward SHG signals from normal and edematous corneas.
Main Results:
- Identified uneven lamellar interspacing and increased thickness in the posterior stroma of edematous corneas.
- Observed no significant changes in the anterior stroma's collagen architecture.
- Demonstrated that backward SHG imaging and signal decay can detect corneal edema.
Conclusions:
- SHG imaging provides detailed morphological insights into corneal edema.
- Structural alterations in edematous corneas are heterogeneous.
- SHG microscopy shows potential for in vivo evaluation of corneal edema.
