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Related Concept Videos

Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
Phosphodiester Linkages01:01

Phosphodiester Linkages

Overview
Phosphodiester bond forms when a phosphoric acid molecule (H3PO4) links with two hydroxyl groups (–OH) of two other molecules, forming two ester bonds. Two water molecules are released in this process. The phosphodiester bond is commonly found in nucleic acids (DNA and RNA) and plays a critical role in their structure and function.
Phosphodiester Bonds Link Nucleotides Together
DNA and RNA are polynucleotides or long chains of nucleotides that are linked together. A nucleotide is...
IP3/DAG Signaling Pathway01:11

IP3/DAG Signaling Pathway

Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and produces two-second...
Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
Phosphorylation01:02

Phosphorylation

The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...

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Related Experiment Video

Updated: Jun 20, 2026

Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters
11:51

Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters

Published on: February 3, 2018

Phosphodiesterase 3 (PDE3): structure, localization and function.

Taku Murata1, Kasumi Shimizu, Kenichi Hiramoto

  • 1Department of Oral and Maxillofacial Surgery, Division of Reparative and Regenerative Medicine, Institute of Medical Science, Mie University Graduate School of Medicine, Tsu, Mie 514-8507, Japan. muratat@clin.medic.mie-u.ac.jp

Cardiovascular & Hematological Agents in Medicinal Chemistry
|August 20, 2009
PubMed
Summary
This summary is machine-generated.

Cyclic nucleotide phosphodiesterases (PDEs) regulate cellular signaling. This review focuses on the PDE3 gene family, exploring its biology, function, and therapeutic potential in cancer and salivary gland treatments.

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Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
10:52

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

Published on: January 6, 2016

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes
07:26

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Published on: October 15, 2016

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Last Updated: Jun 20, 2026

Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters
11:51

Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters

Published on: February 3, 2018

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
10:52

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

Published on: January 6, 2016

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes
07:26

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Published on: October 15, 2016

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Cellular Signaling

Background:

  • Cyclic adenosine 3'5'-monophosphate (cAMP) and cyclic guanosine 3'5'-monophosphate (cGMP) are key intracellular messengers.
  • Cyclic nucleotide phosphodiesterases (PDEs) regulate cAMP and cGMP levels, influencing diverse biological processes.
  • There are at least 11 gene families of PDEs, each with unique regulatory mechanisms.

Purpose of the Study:

  • To provide a general overview of PDEs.
  • To focus on the PDE3 gene family, detailing its molecular biology, structure, and function.
  • To explore the therapeutic potential of PDE3, particularly in malignant tumor cells and salivary gland applications.

Main Methods:

  • Literature review of existing research on PDEs and the PDE3 family.
  • Analysis of molecular biology, structural, and functional data for PDE3.
  • Exploration of preclinical and clinical data regarding PDE3 as a therapeutic target.

Main Results:

  • PDE3 enzymes play a crucial role in regulating intracellular cyclic nucleotide concentrations.
  • The PDE3 gene family exhibits complex regulation and diverse functions.
  • PDE3 inhibition shows promise as a therapeutic strategy for certain cancers and salivary gland disorders.

Conclusions:

  • PDE3 represents a significant target for therapeutic intervention due to its role in cell signaling.
  • Further research into PDE3's specific roles in disease pathogenesis is warranted.
  • Targeting PDE3 may offer novel treatment options for malignant tumors and salivary gland diseases.