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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Related Experiment Video

Updated: Jun 20, 2026

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
09:51

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

Practical, microfabrication-free device for single-cell isolation.

Liang-I Lin1, Shih-Hui Chao, Deirdre R Meldrum

  • 1Center for Ecogenomics, The Biodesign Institute, Arizona State University, Tempe, Arizona, United States of America.

Plos One
|August 22, 2009
PubMed
Summary
This summary is machine-generated.

Researchers developed the Microscale Oil-Covered Cell Array (MOCCA), a low-cost, easy-to-produce device for high-throughput single-cell analysis. This innovative method enables accessible single-cell studies for a wider biology community.

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Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Published on: December 6, 2013

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Last Updated: Jun 20, 2026

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
09:51

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Published on: June 16, 2016

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05:06

Mechanical Dissociation of Tissues for Single Cell Analysis Using a Motorized Device

Published on: November 10, 2023

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation
12:04

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Published on: December 6, 2013

Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell Biology

Background:

  • Microfabricated devices offer potential for cell-level studies but lack accessibility for many researchers.
  • Existing methods often require specialized microengineering expertise and are costly.

Purpose of the Study:

  • Introduce the Microscale Oil-Covered Cell Array (MOCCA) as an accessible, low-cost platform for high-throughput single-cell analysis.
  • Demonstrate MOCCA's ease of production and operation for researchers without microengineering backgrounds.

Main Methods:

  • MOCCA utilizes discrete aqueous droplets separated by oil on patterned hydrophilic surfaces.
  • Cells are isolated at near single-cell levels within 900-picoliter droplets.
  • Escherichia coli bacteria were used to demonstrate cell distribution, fitting a Poisson distribution.

Main Results:

  • MOCCA enables high-throughput single-cell analysis with simplified fabrication.
  • Cell seeding in approximately 3000 droplets was achieved in under 10 minutes.
  • The device significantly reduces costs and enhances efficiency compared to traditional microfabrication.

Conclusions:

  • MOCCA provides a low-cost, user-friendly solution for single-cell analysis.
  • The platform democratizes access to microscale cell array technology for the broader biology community.
  • MOCCA enhances fabrication and operational efficiency for diverse cell-level studies.