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Published on: May 27, 2015
Suppression of CXCR4 expression in mast cells upon IgE-mediated antigen stimulation
Junji Matsuura1, Mariko Sakanaka, Norio Sato
1Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan.
Objective:
Recent studies have demonstrated that a variety of chemokine receptors are expressed in mast cells. We investigated the changes in mRNA expression of CXCRs in murine IL-3-dependent bone marrow-derived mast cells (BMMCs) to clarify how the CXCR expression is regulated in mast cells.
Methods:
Expression of CXCR mRNA was measured by RNase protection assay. Functional expression of CXCRs was confirmed by monitoring intracellular Ca(2+) mobilization.
Results:
CXCR4 mRNA expression was transiently induced in BMMCs in serum-dependent fashion and was completely suppressed upon IgE-mediated antigen stimulation. In contrast, CXCR5 mRNA expression was induced upon IgE-mediated antigen stimulation. Changes in the intracellular Ca(2+) mobilization induced by CXCL12 strongly indicated the functional expression of CXCR4. The decrease in CXCR4 and the increase in CXCR5 mRNA expression was also observed in BMMCs stimulated with thapsigargin, a phorbol ester, and stem cell factor.
Conclusion:
The mRNA expression of CXCR4 is differentially regulated in BMMCs upon various stimuli including IgE-mediated antigen stimulation.
Insights
Chemokine receptor CXCR4 mRNA expression in mast cells is regulated by serum and IgE stimulation, while CXCR5 mRNA increases with IgE. This differential regulation impacts mast cell function.
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- Mast cells express various chemokine receptors.
- Understanding chemokine receptor regulation is crucial for mast cell function.
Purpose of the Study:
- Investigate mRNA expression changes of CXCRs in murine IL-3-dependent bone marrow-derived mast cells (BMMCs).
- Clarify the regulation of CXCR expression in mast cells.
Main Methods:
- RNase protection assay to measure CXCR mRNA expression.
- Intracellular Ca(2+) mobilization assay to confirm functional CXCR expression.
Main Results:
- CXCR4 mRNA was induced by serum and suppressed by IgE stimulation.
- CXCR5 mRNA was induced by IgE stimulation.
- Functional CXCR4 expression was confirmed by CXCL12-induced Ca(2+) mobilization.
Conclusions:
- CXCR4 mRNA expression in BMMCs is differentially regulated by stimuli like IgE.
- CXCR5 mRNA expression is induced by IgE-mediated antigen stimulation.
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