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[Clone and development of RAPD and SCAR markers linked to sex determination in the dioecious species Humulus scandens
Wu Jun Gao1, Fu Cong Sun, Wei Zhi Yin
1College of Life Sciences, Henan Normal University, Henan Xinxiang 453007.
Abstract:
In dioecious plants, the important differences of male and female individuals exist in the genome, so it is very significant to do a comparative study on the sex differences with the DNA markers. In this article, Random Amplification Polymorphic DNA (RAPD) technology was used to increase the Humulus scandens female and male genome teams. The results show that two of the 100 primers, S1519 and S2142, respectly produced a sex-linked band only found in tested males, with the length of 1207 bp and 762 bp. These fragments were cloned and sequenced and then aligned with the GenBank database by BLAST, finding that they are abundant in AT (individually 64%, 54.7%), and no sequence was significantly similar. The RAPD markers were then converted into male-linked SCAR (Sequence Characterized Amplified Regions) markers.
