Related Experiment Video
Updated: Jun 20, 2026
Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary Electrophoresis
Published on: August 22, 2018
Liquid chromatographic analysis of Hg(II) binding by thiol-rich peptides using both UV-vis and electrochemical
Angela Dago1, Olga González-García, Cristina Ariño
1Departament de Química Analítica, Facultat de Química, Universitat de Barcelona, Martí i Franquès, 1-11, E-08028 Barcelona, Spain.
Abstract:
An existing method for HPLC determination of thiol-containing peptides has been successfully adapted to the analysis of mixtures of glutathione (GSH) and some related peptides with their Hg(II) complexes as a first approach to the study of phytochelatin extracts. The separation was achieved in a C18 column with a mobile phase of 0.1% trifluoroacetic acid (TFA) and 0.1% TFA/acetonitrile. Non-derivative UV-vis detection at 202 nm used in the original method has been complemented with amperometric detection at 1.2 V on glassy carbon electrode. Two different Hg(II)-GSH complexes were observed by both detection modes and confirmed by mass spectrometry.
More Related Videos
12:59A Study of the Complexation of Mercury(II) with Dicysteinyl Tetrapeptides by Electrospray Ionization Mass Spectrometry
Published on: January 8, 2016
07:22The Development and Application of Biophysical Assays for Evaluating Ternary Complex Formation Induced by Proteolysis Targeting Chimeras (PROTACS)
Published on: January 12, 2024
