Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular factors associated with lung cancer in people living with HIV.

The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases·2026
Same author

An integrative model of pro- and anti-inflammatory signaling pathways in macrophage differentiation: the role of NF-<i>κ</i>B and CREB.

Frontiers in immunology·2026
Same author

Immune and Virological Factors Influencing Human Respiratory Syncytial Virus Circulation and Increased Prevalence During and After the COVID-19 Pandemic.

Viral immunology·2025
Same author

Modeling uncertainty: the impact of noise in T cell differentiation.

Frontiers in systems biology·2025
Same author

Design, Synthesis, and Biological Activity of Amine-Type Cyclopentanepyridinone Derivatives as HIV‑1 Non-Nucleoside Reverse Transcriptase Inhibitors.

ACS omega·2025
Same author

Molecular characterization of human respiratory syncytial virus in Mexico (season 2023-2024) through whole-genome sequencing.

Scientific reports·2025

Related Experiment Video

Updated: Jun 20, 2026

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay
07:10

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

Published on: September 14, 2014

HIV-envelope-dependent cell-cell fusion: quantitative studies.

Leonor Huerta1, Nayali López-Balderas, Evelyn Rivera-Toledo

  • 1Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, Distrito Federal, CP 04510, México. leonorhh@biomedicas.unam.mx

Thescientificworldjournal
|August 26, 2009
PubMed
Summary

Researchers developed a flow cytometry assay to quantify human immunodeficiency virus (HIV) syncytia formation. This method aids in screening compounds that inhibit HIV-Env-mediated cell fusion, crucial for understanding AIDS pathogenesis.

More Related Videos

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers
11:45

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers

Published on: March 8, 2012

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques
13:13

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques

Published on: September 25, 2018

Related Experiment Videos

Last Updated: Jun 20, 2026

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay
07:10

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

Published on: September 14, 2014

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers
11:45

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers

Published on: March 8, 2012

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques
13:13

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques

Published on: September 25, 2018

Area of Science:

  • Virology
  • Immunology
  • Cell Biology

Background:

  • Human immunodeficiency virus (HIV) infection causes T cell fusion, forming multinucleated syncytia.
  • Syncytia formation is linked to disease progression and AIDS.
  • Fused cells are found in various organs, even during asymptomatic HIV infection.

Purpose of the Study:

  • To develop and validate a flow cytometry method for quantifying HIV-Env-mediated cell fusion.
  • To assess the utility of this assay for screening fusion-inhibiting compounds.
  • To analyze factors influencing syncytia formation and composition.

Main Methods:

  • Utilized lymphocytic cells expressing HIV-envelope (Env) glycoproteins.
  • Employed lipophilic probes and Förster Resonance Energy Transfer (FRET) for fusion detection.
  • Quantified fusion events using flow cytometry.

Main Results:

  • The flow cytometry assay accurately detects and quantifies HIV-Env-mediated cell fusion, including small or rare syncytia.
  • Reaction time and initial cell proportions influence syncytia number, size, and composition.
  • The assay is suitable for screening compounds that modulate HIV-Env-mediated cell fusion.

Conclusions:

  • A novel flow cytometry assay enables direct quantification of HIV-induced cell fusion.
  • Understanding syncytia heterogeneity is vital for comprehending HIV pathogenesis and immune response.
  • This method facilitates the discovery of potential therapeutics targeting HIV syncytia formation.