Related Experiment Video
Updated: Jun 20, 2026
09:39
Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
Published on: December 20, 2014
SUMO-dependent regulation of centrin-2
1Department of Cell Biology, Max-Planck Institute of Biochemistry, D-82152 Martinsried, Germany. uklein@biochem.mpg.de
Journal of Cell Science
|August 27, 2009
Summary
Centrin-2
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Centrins are conserved Ca(2+)-binding proteins crucial for cellular functions.
- Centrin-2 is a key component of centrosomes and involved in DNA repair via the XPC complex.
- Regulation of centrin-2's subcellular localization was previously unknown.
Purpose of the Study:
- To investigate the regulatory mechanisms governing centrin-2's subcellular distribution.
- To identify post-translational modifications affecting centrin-2 localization.
- To elucidate the role of SUMOylation in centrin-2 nuclear import.
Main Methods:
- In vitro and in vivo SUMOylation assays.
- Identification of SUMO E3 ligase (PC2/hPC2) for centrin-2.
- Analysis of centrin-2 localization upon interference with the SUMOylation pathway.
- XPC protein depletion experiments.
Main Results:
- Centrin-2 is a substrate for SUMOylation, primarily by SUMO2/3.
- Human polycomb protein 2 (PC2/hPC2) is identified as the E3 ligase for centrin-2.
- Disruption of SUMOylation causes centrin-2 mislocalization from the nucleus to the cytoplasm.
- SUMOylation enhances centrin-2 binding to the XPC protein.
Conclusions:
- Nucleocytoplasmic shuttling of centrin-2 is regulated by the SUMOylation system.
- Nuclear localization of centrin-2 is dependent on its interaction with the XPC complex.
- SUMOylation is a critical regulator of centrin-2's function in DNA repair.
Related Concept Videos
02:25Centrosome Duplication
The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
02:25Centrosome Duplication
The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
01:45Regulation of Nuclear Protein Sorting
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
02:30Histone Variants at the Centromere
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3 variants are also...
02:14GTPases and their Regulation
Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒ small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins, also known...
Large G-proteins, also known...
02:14GTPases and their Regulation
Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒ small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins, also known...
Large G-proteins, also known...