Related Experiment Video
Updated: Jun 20, 2026

Comparative Analysis of Automatic Fecal Analyzer versus Direct Wet Smear Microscopy for Detecting Parasitic Infections in Stool Samples
Published on: April 25, 2025
Giardia intestinalis assemblages A and B infections in Nepal
Anjana Singh1, Lalitha Janaki, William A Petri
1Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia, Charlottesville, Virginia, USA. anjanas67@hotmail.com
Abstract:
Giardia intestinalis is comprised of two major genotypes, A and B, which may vary in their propensity to cause disease. We tested for the presence of these two genotypes in stool samples from patients with gastrointestinal symptoms in Nepal. A total of 1,096 clinical specimens were screened by microscopy, and 45 samples with G. intestinalis were identified. Giardia infection was confirmed in 35 of 45 samples by a Giardia specific real-time polymerase chain reaction (PCR) assay. Genotyping of the Giardia PCR product by restriction fragment length polymorphism indicated that 74% (26 of 35) were assemblage B, 20% (7 of 35) were assemblage A, and 6% (2 of 35) were mixed assemblages.
Related Concept Videos
Giardiasis
Amebiasis
Fungal Phylum Microsporidia
Bacterial Gastroenteritis
Poliomyelitis
Trichomoniasis
