Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nan-Nong 999: a new wheat cultivar combining FHB resistance and high yield developed through molecular design breeding.

Molecular breeding : new strategies in plant improvement·2026
Same author

Baicalin attenuates angiotensin II-induced vascular endothelial injury via targeted activation of the Nrf2 signaling pathway: Integrated computational and in vitro evidence.

Molecular diversity·2026
Same author

Comprehensive exploration of the role of multimodal programmed cell death- associated lncRNAs in the prognosis and immunity of glioma.

Frontiers in immunology·2026
Same author

Two Multidrug-Resistant <i>Escherichia coli</i> Isolates from Blood Cultures After Cervical Conization in a Patient with Cervical Intraepithelial Neoplasia Grade II: Phenotypic and Genomic Characterization.

Pathogens (Basel, Switzerland)·2026
Same author

An X-linked sex determination mechanism in cannabis and hop.

Nature communications·2026
Same author

Global survey of extraintestinal pathogenic Escherichia coli identifies stable serogroup-virulence-resistance linkages.

Genome medicine·2026
Same journal

Geographic distribution of sex chromosome polymorphism in Anastrepha fraterculus sp. 1 from Argentina.

BMC genetics·2020
Same journal

Development and characterization of a pupal-colour based genetic sexing strain of Anastrepha fraterculus sp. 1 (Diptera: Tephritidae).

BMC genetics·2020
Same journal

Improvement on the genetic engineering of an invasive agricultural pest insect, the cherry vinegar fly, Drosophila suzukii.

BMC genetics·2020
Same journal

Precise single base substitution in the shibire gene by CRISPR/Cas9-mediated homology directed repair in Bactrocera tryoni.

BMC genetics·2020
Same journal

Climate stress resistance in male Queensland fruit fly varies among populations of diverse geographic origins and changes during domestication.

BMC genetics·2020
Same journal

Genetic structure and symbiotic profile of worldwide natural populations of the Mediterranean fruit fly, Ceratitis capitata.

BMC genetics·2020
See all related articles

Related Experiment Video

Updated: Jun 20, 2026

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
07:08

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

Published on: July 14, 2015

Single-feature polymorphism discovery by computing probe affinity shape powers.

Wayne Wenzhong Xu1, Seungho Cho, S Samuel Yang

  • 1Supercomputing Institute for Advanced Computational Research, University of Minnesota, Minnesota, MN 55455, USA. wxu@msi.umn.edu

BMC Genetics
|August 28, 2009
PubMed
Summary
This summary is machine-generated.

A new computing method enhances single-feature polymorphism (SFP) discovery, improving specificity and sensitivity. This approach identifies DNA polymorphisms more effectively and provides valuable genetic markers for crop improvement.

More Related Videos

Pharmacophore Modeling for Targets with Extensive Ligand Libraries: A Case Study on SARS-CoV-2 Mpro
05:50

Pharmacophore Modeling for Targets with Extensive Ligand Libraries: A Case Study on SARS-CoV-2 Mpro

Published on: September 26, 2025

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Related Experiment Videos

Last Updated: Jun 20, 2026

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
07:08

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

Published on: July 14, 2015

Pharmacophore Modeling for Targets with Extensive Ligand Libraries: A Case Study on SARS-CoV-2 Mpro
05:50

Pharmacophore Modeling for Targets with Extensive Ligand Libraries: A Case Study on SARS-CoV-2 Mpro

Published on: September 26, 2025

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Area of Science:

  • Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • Single-feature polymorphism (SFP) discovery is a rapid DNA polymorphism identification method.
  • Existing SFP detection methods suffer from high false positive rates or low sensitivity.
  • A novel computational approach for SFP discovery is presented.

Purpose of the Study:

  • To develop a new computing method for enhanced SFP discovery.
  • To improve the specificity and sensitivity of SFP detection.
  • To provide a user-friendly tool for SFP analysis.

Main Methods:

  • Developed a computational method calculating SFP weight scores based on probe affinity differences and shape powers.
  • Validated the method using known sequence information and compared it with existing approaches.
  • Implemented a web application for SFP detection.

Main Results:

  • Identified 364 SFPs in a barley near-isogenic line pair (wild type vs. mutant uniculm2 allele).
  • Localized most SFP polymorphisms to chromosome 6H near the Cul2 locus.
  • Demonstrated superior specificity and sensitivity compared to previous methods.

Conclusions:

  • The new SFP discovery method offers improved performance over existing techniques.
  • The method is applicable to other organisms utilizing GeneChip technology.
  • The identified SFPs serve as genetic markers for fine mapping and cloning the Cul2 locus in barley.