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Determination of airborne particle-associated benz[a]anthracene-7,12-quinone using high-performance liquid
Takayuki Kameda1, Tomoko Goto, Akira Toriba
1Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University Kakuma-machi, Kanazawa, Ishikawa 920-1192, Japan. kameda@p.kanazawa-u.ac.jp
Abstract:
A simple and sensitive method for measuring airborne particle-associated benz[a]anthracene-7,12-quinone (BaAQ) based on two-dimensional high-performance liquid chromatography (HPLC) with fluorescence detection was established. The system involves an ODS column for sample clean up, a 6-port switching valve, an ODS column for trapping the fraction containing BaAQ, an ODS column for sample separation, and a Pt-Rh catalytic column for reduction of BaAQ to a corresponding fluorescent compound. The accuracy of the assay, as applied to airborne particulate sample extracts spiked with known amounts of BaAQ was 94-108%. The detection limit was 97 fmol per injection (signal-to-noise ratio=3), and the calibration range was from 1 to 100 pmol with excellent proportionality (R(2)=0.9995). BaAQ in airborne particles collected in Osaka, Japan every 3 h was successfully measured using the established analytical method with simple sample preparation steps: ultrasonic extraction in organic solvent and concentration under reduced pressure and/or nitrogen stream.
