Related Experiment Video
Updated: Jun 20, 2026

DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells
Published on: March 20, 2021
Interfacial behavior of a hairpin DNA probe immobilized on gold surfaces
M Steichen1, N Brouette, C Buess-Herman
1Service de Chimie Analytique et Chimie des Interfaces, Faculté des Sciences, Université Libre de Bruxelles, Boulevard du Triomphe, CP 255, 1050 Bruxelles, Belgique.
Abstract:
Neutron reflection has been used in combination with electrochemical and quartz microbalance techniques to characterize a mixed monolayer of thiolated hairpin ss-DNA and 4-mercaptobutan-1-ol monolayer self-assembled on gold before and after hybridization with a cDNA target. Neutron reflection has revealed the opening of the stem-loop configuration of the probe associated with helix formation. This change in conformation correlates to the modification of the electron-transfer resistance associated with the [Fe(CN)6](3-/4-) redox marker present in solution.
Related Concept Videos
Labeling DNA Probes
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Single-Strand DNA Binding Proteins
Protein-protein Interfaces

