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Related Concept Videos

Reproductive Cloning01:27

Reproductive Cloning

Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
Reproductive Cloning01:27

Reproductive Cloning

Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
Cloning of Dolly the Sheep01:08

Cloning of Dolly the Sheep

The first successfully cloned mammal was Dolly, a sheep, born on 5th July 1996 at Roslin Institute, Scotland. The cloned sheep was named after the American singer Dolly Parton. Dolly lived for seven years and died of respiratory complications, which is speculated to be due to the actual age of her DNA. Because the DNA in cloned cells belongs to an older individual,  the cloned individual’s life expectancy may be affected. Indeed, analysis of Dolly’s DNA revealed shorter telomeres than other...
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Transgenic Organisms

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Related Experiment Video

Updated: Jun 20, 2026

Functional Cloning Using a Xenopus Oocyte Expression System
09:40

Functional Cloning Using a Xenopus Oocyte Expression System

Published on: January 30, 2016

ORFeome cloning.

Osamu Ohara1

  • 1Department of Human Genome Research, Kazusa DNA Research Institute, Chiba, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|September 1, 2009
PubMed
Summary

Reverse chemical genomics accelerates drug discovery. Preparing genome-wide ORFeome clones is crucial for this approach, and this chapter reviews available technologies and provides selection guidelines.

Area of Science:

  • Genomics
  • Chemical Biology
  • Molecular Biology

Background:

  • Reverse chemical genomics offers a powerful strategy for discovering novel compounds that modulate biological phenotypes.
  • The success of this approach hinges on the availability of well-prepared research resources, including comprehensive clone collections.
  • ORFeome clones, which enable the production of proteins in native or fusion forms, are essential for advancing reverse chemical genomics.

Purpose of the Study:

  • To provide an overview of currently available ORFeome cloning technologies.
  • To offer a selection guideline for choosing appropriate ORFeome cloning methods.
  • To facilitate the broader adoption and implementation of reverse chemical genomics.

Main Methods:

  • Review of existing ORFeome cloning technologies.

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A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning
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A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning

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Related Experiment Videos

Last Updated: Jun 20, 2026

Functional Cloning Using a Xenopus Oocyte Expression System
09:40

Functional Cloning Using a Xenopus Oocyte Expression System

Published on: January 30, 2016

Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit
06:28

Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit

Published on: September 2, 2025

A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning
10:52

A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning

Published on: October 30, 2012

  • Comparative analysis of different cloning platforms.
  • Development of criteria for technology selection.

    • Main Results:

    • Identification and categorization of various ORFeome cloning strategies.
    • Presentation of a decision-making framework for technology selection.
    • Highlighting the importance of ORFeome clone preparation.

    Conclusions:

    • The strategic preparation of ORFeome clones is fundamental to the advancement of reverse chemical genomics.
    • Understanding available cloning technologies and their selection criteria is key to efficient compound discovery.
    • This work provides valuable guidance for researchers aiming to utilize reverse chemical genomics.