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Paraspeckles: nuclear bodies built on long noncoding RNA
1School of Biomedical, Biomolecular, and Chemical Sciences, University of Western Australia, Crawley, Western Australia 6009, Australia.
The Journal of Cell Biology
|September 2, 2009
Summary
Paraspeckles are nuclear bodies regulating gene expression via RNA retention. Core proteins and NEAT1 long noncoding RNA form these structures, potentially serving as a model for other RNA-based nuclear bodies.
Area of Science:
- Cell Biology
- Molecular Biology
- Genetics
Background:
- Paraspeckles are nuclear ribonucleoprotein bodies in mammalian cells.
- They regulate gene expression through nuclear RNA retention.
- Core proteins (PSF/SFPQ, P54NRB/NONO, PSPC1) and NEAT1 lncRNA are key components.
Purpose of the Study:
- To elucidate the composition and function of paraspeckles.
- To understand the role of NEAT1 long noncoding RNA in paraspeckle formation.
- To propose paraspeckles as a model for lncRNA-based nuclear bodies.
Main Methods:
- Analysis of core paraspeckle protein interactions.
- Investigation of NEAT1 long noncoding RNA's role in paraspeckle integrity.
- Comparative analysis with other discovered long noncoding transcripts.
Main Results:
- Paraspeckles are formed by the association of DBHS proteins and NEAT1 RNA.
- These structures are crucial for maintaining nuclear RNA homeostasis.
- NEAT1 is essential for paraspeckle assembly and stability.
Conclusions:
- Paraspeckles are essential nuclear bodies regulated by NEAT1 lncRNA and DBHS proteins.
- They play a significant role in gene expression control in differentiated cells.
- Paraspeckles represent a paradigm for understanding lncRNA-mediated formation of subnuclear bodies.
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