Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
The DNA Helix01:16

The DNA Helix

Overview
The DNA Helix01:07

The DNA Helix

Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA is composed of two strands of nucleotides that wind around each other to form a spring-like structure called a double helix. However, the double helix is not perfectly symmetrical. Instead, there are regularly occurring grooves in the structure. The major groove occurs where the sugar-phosphate backbones are relatively far apart. This space...
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
Karyotyping01:17

Karyotyping

Describing the number and physical features of chromosomes can reveal abnormalities that underlie genetic diseases. This description is facilitated by special staining techniques that produce a particular banding pattern on each chromosome. State-of-the-art techniques make this approach even more powerful, enabling the detection of individual genes that cause disease.A Simple Chromosome Staining Technique Provides Valuable Scientific InsightSome genetic diseases can be detected by looking at...
DNA as a Genetic Template02:05

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of switching high-sensitivity cardiac troponin assays on risk stratification in suspected acute coronary syndrome.

European heart journal. Acute cardiovascular care·2026
Same author

Clinical Decisions and Outcomes After Switching High-Sensitivity Cardiac Troponin Assays in Suspected ACS: An Interrupted Time-Series Study.

JAMA cardiology·2025
Same author

Mechanism for a molecular assembler of sequence-controlled polymers using parallel DNA and a DNA polymerase.

Nanoscale horizons·2025
Same author

Controlling DNA-RNA strand displacement kinetics with base distribution.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Tissue-specific modulation of CRISPR activity by miRNA-sensing guide RNAs.

Nucleic acids research·2025
Same author

Coarse-grained modeling of DNA-RNA hybrids.

The Journal of chemical physics·2024
Same journal

Carbonylative Aminative Suzuki-Miyaura Coupling: Pd-Catalyzed Synthesis of Amides from Vinyl/Aryl Halides and Boronic Acids.

Journal of the American Chemical Society·2026
Same journal

Divergent Asymmetric Synthesis of Glutinosasins A-E.

Journal of the American Chemical Society·2026
Same journal

Ultrastrong Polyketone Hot-Melt Adhesives Enabled by Ni-Catalyzed Carbonylative Polymerization.

Journal of the American Chemical Society·2026
Same journal

Programmable Anomalous Photovoltaics Enabled by Light-Electric Dual-Field Control.

Journal of the American Chemical Society·2026
Same journal

Biomimetic Redox-Mediated Proton Relay in Nanoreactors for Photocatalysis.

Journal of the American Chemical Society·2026
Same journal

The Sulfur Monoxide-Water Complex.

Journal of the American Chemical Society·2026
See all related articles

Related Experiment Video

Updated: Jun 20, 2026

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray
09:05

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

A two-dimensional DNA array: the three-layer logpile.

Jonathan Malo1, James C Mitchell, Andrew J Turberfield

  • 1Clarendon Laboratory, Department of Physics, University of Oxford, Parks Road, Oxford OX1 3PU, UK.

Journal of the American Chemical Society
|September 3, 2009
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel three-layer logpile (3LL) DNA array for enhanced structural order in synthetic crystals. This DNA self-assembly method creates extensive, ordered sheets, paving the way for advanced materials.

More Related Videos

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
09:32

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules
10:45

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

Published on: June 20, 2020

Related Experiment Videos

Last Updated: Jun 20, 2026

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray
09:05

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
09:32

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules
10:45

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

Published on: June 20, 2020

Area of Science:

  • Synthetic Biology
  • Nanotechnology
  • Biophysics

Background:

  • DNA self-assembly offers a route to creating ordered nanostructures.
  • Existing DNA arrays can have limitations in structural integrity and order.
  • Periodic templates are crucial for applications like synthetic protein crystallization.

Purpose of the Study:

  • To describe the design and self-assembly of a novel three-layer logpile (3LL) DNA array.
  • To create a DNA array with maximized structural order and continuous helices.
  • To characterize the assembly process and the resulting nanostructure.

Main Methods:

  • Design of a two-dimensional DNA array using four synthetic oligonucleotides and a Holliday junction motif.
  • UV absorbance measurements to study the assembly kinetics and hysteresis.
  • Negative-stain transmission electron microscopy (TEM) for structural visualization.
  • Iterative correlation mapping and averaging of TEM micrographs.

Main Results:

  • Successful self-assembly of the three-layer logpile (3LL) DNA array.
  • Observation of rate-dependent hysteresis during 3LL assembly via UV absorbance.
  • Formation of extensive sheets (micrometer-scale) of 3LL structures observed by TEM.
  • Averaged TEM projection image validated the computer-generated model of the crystal structure.

Conclusions:

  • The three-layer logpile (3LL) is a stable, two-dimensional DNA array with high structural order.
  • The continuous helix design enhances the potential for ordered templating.
  • This DNA array represents a significant advancement for applications in synthetic materials and crystallography.