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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
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Microfluidic concentration-enhanced cellular kinase activity assay.

Jeong Hoon Lee1, Benjamin D Cosgrove, Douglas A Lauffenburger

  • 1Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.

Journal of the American Chemical Society
|September 3, 2009
PubMed
Summary
This summary is machine-generated.

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This study presents a micro/nanofluidic chip for enhanced cell kinase assays. The device significantly boosts reaction speed and sensitivity using electrokinetic trapping, enabling faster diagnostics.

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Cell Biology

Background:

  • Cell kinase assays are crucial for understanding cellular functions and disease.
  • Traditional assays often require large sample volumes and long incubation times.
  • Improving assay sensitivity and speed is essential for diagnostics and systems biology.

Purpose of the Study:

  • To develop a simple, disposable micro/nanofluidic chip for preconcentration-enhanced cell kinase assays.
  • To improve reaction velocity, sensitivity, and reduce assay time.
  • To enable single-cell level analysis for diagnostics and systems biology.

Main Methods:

  • Fabrication of a polydimethylsiloxane (PDMS) micro/nanofluidic chip.
  • Utilizing electrokinetic trapping for preconcentration of analytes from cell lysate.

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  • Performing in vitro cell kinase assays using the preconcentration chip.

    • Main Results:

    • Achieved at least a 25-fold increase in reaction velocity.
    • Demonstrated a 65-fold enhancement in assay sensitivity.
    • Reduced assay time to less than 10 minutes with minimal sample volume (approx. 5 cells).

    Conclusions:

    • The developed micro/nanofluidic chip offers a powerful tool for concentration-enhanced cell kinase assays.
    • The device enables rapid, sensitive, and low-volume analysis suitable for single-cell studies.
    • Potential applications include diagnostics and systems biology research, particularly when integrated with cell culture microdevices.