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A highly attenuated measles virus vaccine strain encodes a fully functional C protein

Yuichiro Nakatsu1, Makoto Takeda, Masaharu Iwasaki

  • 1Department of Virology, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.

Journal of Virology
|September 4, 2009
PubMed

Insights

Measles virus C protein function was analyzed independently. Results show the C protein is functional in a vaccine strain, suggesting P or V proteins contribute to measles attenuation.

Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Measles virus (MeV) P, V, and C proteins share overlapping reading frames within the P gene.
  • Analyzing individual protein functions during MeV infection is challenging due to this overlap.

Purpose of the Study:

  • To develop a system for independent analysis of the MeV C protein.
  • To investigate the role of the C protein in MeV pathogenesis and attenuation.

Main Methods:

  • Constructed a modified MeV genome with the C protein gene in a separate transcription unit.
  • Utilized this system to express and analyze the C protein from an attenuated vaccine strain.

Main Results:

  • The Edmonston lineage vaccine strain encodes a fully functional C protein.
  • The P and/or V proteins, not C, are implicated in the attenuated phenotype of this MeV strain.

Conclusions:

  • The developed system enables independent functional studies of MeV C protein.
  • MeV attenuation is likely mediated by the P and/or V proteins, not a non-functional C protein.

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